अमूर्त

Study of difference of peripheral blood lymphocytes immunophenotyping and NK cells on connective tissue disease combined with interstitial lung disease

Dandan Zhang, Yansheng Wang, Yan Rong, Xiaoli Li, Jian Xu, Bing Han, Junli Wang

Objective: To investigate the clinical value of peripheral blood lymphocytes immunophenotyping and Natural Killer cells (NK) in the judgment of the types of Connective Tissue Disease Combined with Interstitial Lung Disease (CTD-ILD).

Methods: 262 CTD-ILD patients and 50 healthy volunteers in our hospital from January 2010 to December 2015 were enrolled into the study. There were 164 patients with CTD-ILD and 98 patients with ILD. The clinical symptoms and High Resolution CT (HRCT) of all patients were observed and compared between the two groups. The serum levels of CD3+, CD4+, CD8+, CD19+, CD56+ T cells were detected by flow cytometry and compared among the three groups.

Results: There were no difference of the clinical symptoms and the characteristics of HRCT between the two patients groups (P>0.05). The serum level of CD3+-CD8+ T cells of patients in the CTD-ILD group was higher than the control group and ILD group (P<0.05); and the serum level of CD3-CD56+ T cells and the ratio of CD4+/CD8+ in the CTD-ILD group was lower than the control group and ILD group (P<0.05). But there was no difference of the peripheral blood lymphocytes immunophenotyping between the ILD group and control group (P>0.05).

Conclusion: The immunophenotype of peripheral blood lymphocytes could be helpful to judge the types of connective tissue disease combined with interstitial lung disease.